Objective To determine the vitamin C content in commercial vitamin C tablets by titration between vitamin C (ascorbic acid) & iodine solution. Hence, compare this data with the manufacturer's specification. Principle In this analysis, certain amount of iodine solution, which is known in excess, is added to acidified ascorbic acid solution. Brown iodine can be easily reduced by acidified ascorbic acid to form colourless iodide ion: �� Equation I The excess iodine is then back titrated by standard sodium thiosulphate solution, by using a burette and starch solution (used as end point indicator) Similarly, iodine can also be decolourized by thiosulphate ion: 2S2O32- + I2 �� S4O62- + 2I- �� Equation II Any iodine present will react with starch to form a blue-black complex. However, when all available iodine has been reacted, the blue-black complex becomes colourless which signals the end-point. Hence, the mass of asc......

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